I wouldn't call such things "mature."
A "mature" theme in a game would be having your character cope with the fact that his/her wife/husband is going to die soon, and there is nothing the doctors can do about it, as they have contracted a rare form of a non-lethal disease...but it just so happens that this particular, extremely rare strain is deadly.
A singing mountain of poop and running around with a minigun lighting everything up isn't "mature." Fun? Hell yes! But not mature. Sophamoric at best.
There! I feel much better.
In the Japanese version of Final Fight, when you smack Poison in the face and she recoils, her shirt flys up and can see her 16-bit boobies.
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