I got about that far down once when I was trying to see a difference, it was harrowing and not fun and the results were inconclusive at best. Just found 2 more Fire Seeds and I dont want to waste them but I cant upgrade the normal one past +10. Also just realized the max Estus Flasks you can have on the first game is 12, but I have extra shards so Im guessing maybe you can upgrade further on NG+?
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